Superoxide dismutase in the anal gills of the mosquito larvae of Aedes aegypti: its inhibition by alpha-terthienyl

Arch Insect Biochem Physiol. 1991;16(4):249-55. doi: 10.1002/arch.940160404.

Abstract

The anal gills of the mosquito larvae of Aedes aegypti were shown to possess superoxide dismutase (EC 1.15.1.1) activity, which increased with the maturation of the larvae from instar 1 to instar 4. This enzyme was highly inhibited upon treatment of the larvae with alpha-terthienyl (2,2':5,2"-terthiophene) and subsequent exposure to long-wave ultraviolet light. Inhibition also occurred with treatment of the crude enzyme extract in a similar fashion. Exposure of the enzyme to the ultraviolet light alone or the treatment of the enzyme with alpha-terthienyl in darkness could not manifest this inhibition. This finding adds a new dimension to the complex mechanism(s) proposed for the photodynamic toxicity of alpha-terthienyl.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / enzymology*
  • Aedes / growth & development
  • Analysis of Variance
  • Animals
  • Digestive System / enzymology
  • Kinetics
  • Larva
  • Superoxide Dismutase / antagonists & inhibitors
  • Superoxide Dismutase / metabolism*
  • Thiophenes / pharmacology*

Substances

  • Thiophenes
  • alpha-terthienyl
  • Superoxide Dismutase