Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice

Brain Res. 2007 Dec 14;1185:239-45. doi: 10.1016/j.brainres.2007.07.103. Epub 2007 Oct 10.

Abstract

Photochemically induced cerebral infarction has been considered a clinically relevant model for ischemic stroke. We evaluated various transgenic mice to study the role of platelet adhesion molecules in this model. Infarction to the sensorimotoric cortex was induced by erythrosin B and laser light. Infarct volumes were calculated from triphenyltetrazolium chloride stained brain slices. Thrombus formation and vessel leakage were observed in vivo by multiphoton microscopy. Mice mutant in VWF, GPIbalpha, beta3 integrin, and P-selectin did not show any significant differences in infarct volume compared to wild type (WT). This is in contrast to the intraluminal middle cerebral artery occlusion model in which alphaIIbbeta3 integrin, GPIbalpha, and P-selectin are known to modulate infarct size. Multiphoton microscopy showed that small, non-occlusive embolizing platelet thrombi formed in the photochemically injured brains. Massive vessel leakage was observed within 25 min of laser injury. Interestingly, we observed a significant increase in infarct size with aging, accordant with heightened fragility of the blood brain barrier (BBB) in older mice. This model of photochemically induced stroke is closer to a BBB injury model than a thrombotic stroke model in which platelets and their adhesion molecules are crucial. This model will be useful to study mechanisms regulating BBB permeability.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Age Factors
  • Animals
  • Antigens / genetics
  • Brain Infarction / etiology
  • Brain Infarction / metabolism*
  • Brain Infarction / pathology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / physiology*
  • Disease Models, Animal
  • Integrin beta3 / genetics
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • P-Selectin / genetics
  • Photochemistry / methods*
  • Receptors, Cell Surface / deficiency
  • Stroke / complications*
  • Stroke / etiology*
  • Time Factors
  • von Willebrand Factor / immunology

Substances

  • Antigens
  • Cell Adhesion Molecules
  • Il4ra protein, mouse
  • Integrin beta3
  • P-Selectin
  • Receptors, Cell Surface
  • Von Willebrand antigen
  • von Willebrand Factor