High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3

Plant Cell Rep. 2008 Mar;27(3):471-8. doi: 10.1007/s00299-007-0472-y. Epub 2007 Nov 13.


Brachypodium distachyon (Brachypodium) is a small grass with biological attributes (rapid generation time, small genome, diploid accessions, small stature and simple growth requirements) that make it suitable for use as a model system. In addition, a growing list of genomic resources have been developed or are currently under development including: cDNA libraries, BAC libraries, EST sequences, BAC end sequences, a physical map, genetic markers, a linkage map and, most importantly, the complete genome sequence. To maximize the utility of Brachypodium as a model grass it is necessary to develop an efficient Agrobacterium-mediated transformation system. In this report we describe the identification of a transformable inbred diploid line, Bd21-3, and the development of a transformation method with transformation efficiencies as high as 41% of co-cultivated calluses producing transgenic plants. Conducting the co-cultivation step under desiccating conditions produced the greatest improvement in transformation efficiency.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Blotting, Southern
  • DNA, Plant / genetics
  • Genome, Plant / genetics
  • Plants, Genetically Modified
  • Poaceae / genetics*
  • Rhizobium / genetics*
  • Sequence Analysis, DNA
  • Transformation, Genetic / genetics*


  • DNA, Plant