Identification of human T cell targets recognized during Chlamydia trachomatis genital infection

J Infect Dis. 2007 Nov 15;196(10):1546-52. doi: 10.1086/522524. Epub 2007 Oct 31.


The specificity of the human T cell response to Chlamydia trachomatis was investigated by stimulating lymphocytes from 16 case patients with urogenital infection by use of a size-fractionated serovar D lysate. Considerable heterogeneity was found among case patients, and multiple protein fractions were recognized in each specimen. Mass spectrometry analysis of the 30-42-kDa T cell-stimulating region identified 10 C. trachomatis proteins. Of these, CT583, CT603, and CT610 were identified as strong antigens that induced significantly higher levels of IFN- gamma secretion in PBMCs from case patients, compared with PBMCs from control donors. All 3 proteins were recognized in specimens from case patients infected with serovars D-F, the most prevalent serovars. McDonald-Kreitman and Tajima's D tests involving clinical isolates from the same samples showed evidence for frequency-dependent selection on ct583. We predict that CT583 is a target of acquired protective immune responses in humans.

MeSH terms

  • Case-Control Studies
  • Chlamydia Infections / blood
  • Chlamydia Infections / immunology*
  • Chlamydia Infections / microbiology
  • Chlamydia trachomatis / genetics
  • Chlamydia trachomatis / immunology*
  • DNA Primers
  • DNA, Bacterial / analysis
  • Humans
  • Polymerase Chain Reaction
  • T-Lymphocytes / physiology*


  • DNA Primers
  • DNA, Bacterial