Effects of oral administration of aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem on some testicular function indices of male rats

J Ethnopharmacol. 2008 Jan 17;115(2):288-92. doi: 10.1016/j.jep.2007.10.004. Epub 2007 Oct 9.


Aim of the study: The effects of administration of aqueous extract of Fadogia agrestis (Schweinf. Ex Hiern) stem on some testicular function indices of male rats (Rattus norvegicus) and their recovery potentials for 10 days were investigated.

Materials and methods: Rats were grouped into four: A, B, C and D where A (the control) received orally 1 ml of distilled water (the vehicle), B, C and D (the test groups) received orally on daily basis graded doses of 18, 50 and 100mg/kg body weight of the plant extract, respectively, for 28 days.

Results: Compared with the control, extract administration for 28 days at all the doses resulted in significant increase (P<0.05) in percentage testes-body weight ratio, testicular cholesterol, sialic acid, glycogen, acid phosphatase and gamma-glutamyl transferase activities while there was significant decrease (P<0.05) in the activities of testicular alkaline phosphatase, acid phosphatase, glutamate dehydrogenase and concentrations of protein. Recoveries were made by the animals on some of the testicular function indices mainly at 18 mg/kg body weight.

Conclusions: The alterations brought about by the aqueous extract of Fadogia agrestis stem are indications of adverse effects on the male rat testicular function and this may adversely affect the functional capacities of the testes. The recovery made at the dose of 18 mg/kg body weight as used in folklore medicine suggests that it does not exhibit permanent toxicity at this dose.

MeSH terms

  • Acid Phosphatase / drug effects
  • Acid Phosphatase / metabolism
  • Administration, Oral
  • Alkaline Phosphatase / drug effects
  • Alkaline Phosphatase / metabolism
  • Animals
  • Body Weight / drug effects
  • Cholesterol / metabolism
  • Dose-Response Relationship, Drug
  • Glutamate Dehydrogenase / drug effects
  • Glutamate Dehydrogenase / metabolism
  • Glycogen / metabolism
  • Male
  • N-Acetylneuraminic Acid / metabolism
  • Nigeria
  • Plant Extracts / administration & dosage
  • Plant Extracts / toxicity*
  • Proteins / drug effects
  • Proteins / metabolism
  • Rats
  • Rubiaceae / chemistry*
  • Testis / drug effects*
  • Testis / metabolism
  • gamma-Glutamyltransferase / drug effects
  • gamma-Glutamyltransferase / metabolism


  • Plant Extracts
  • Proteins
  • Glycogen
  • Cholesterol
  • Glutamate Dehydrogenase
  • gamma-Glutamyltransferase
  • Alkaline Phosphatase
  • Acid Phosphatase
  • N-Acetylneuraminic Acid