Efficient quenching of oligomeric fluorophores on a DNA backbone

J Am Chem Soc. 2007 Dec 19;129(50):15426-7. doi: 10.1021/ja075968a. Epub 2007 Nov 21.

Abstract

The quenching properties of a series of oligodeoxyribosides bearing fluorophore ‘bases’ is described. Sequences of adjacent, π-stacked pyrenes exhibit stronger electronic interactions visible in both absorbance and emission spectra than pyrenes that are insulated by intervening adenines. Quenching by N, N′-dimethyl-4,4′-bipyridinium dichloride is efficient for excimer-and exciplex-forming oligomers, with Stern-Volmer constants comparable to conjugated polymer “superquenching” schemes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • DNA