Analysis of molecular inversion probe performance for allele copy number determination

Genome Biol. 2007;8(11):R246. doi: 10.1186/gb-2007-8-11-r246.


We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 mug) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alleles*
  • Cell Line, Tumor
  • Chromosome Aberrations
  • Chromosome Inversion*
  • False Positive Reactions
  • Humans
  • Loss of Heterozygosity
  • Molecular Probes*
  • Polymerase Chain Reaction
  • ROC Curve


  • Molecular Probes