Effects of caffeic acid phenethyl ester on pancreatitis in rats

J Surg Res. 2008 Mar;145(1):19-24. doi: 10.1016/j.jss.2007.04.019. Epub 2007 Oct 29.


Background: This study investigated the effect of caffeic acid phenethyl ester (CAPE) on acute necrotizing pancreatitis (ANP) induced by glycodeoxycholic acid in rats. CAPE, an active component of honeybee propolis, has previously been determined to have antioxidant, anti-inflammatory, antiviral, and anticancer activities.

Materials and methods: Forty-eight rats were divided into four groups of 12. Group 1 animals received intraductal saline and intravenous saline infusion treatment. Group 2 was given intraductal saline and intraperitoneal CAPE infusion treatment. ANP was induced in the animals in group 3 (ANP with saline infusion), and group 4 had induced ANP plus CAPE infusion treatment (ANP with CAPE infusion). Sampling was performed 48 h after treatment.

Results: ANP induction significantly increased mortality rate, pancreatic necrosis, and bacterial infection in pancreatic and extrapancreatic organs. ANP also increased levels of amylase and alanine aminotransferase (ALT) in serum, increased levels of urea and lactate dehydrogenase in bronchoalveolar lavage fluid (BAL LDH), increased the activities of myeloperoxidase (MPO) and malondialdehyde (MDA) in pancreas and lung tissue, and decreased the serum calcium levels. The use of CAPE did not significantly reduce the mortality rate but significantly reduced the ALT and BAL LDH levels, the activities of MPO and MDA in the pancreas, the activity of MDA in the lungs, and pancreatic damage. The administration of CAPE did not reduce the bacterial infection.

Conclusions: These results indicate that CAPE had beneficial effects on the course of ANP in rats and suggest that CAPE shows promise as a treatment for ANP.

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Bacterial Translocation / drug effects
  • Bronchoalveolar Lavage Fluid
  • Caffeic Acids / pharmacology
  • Caffeic Acids / therapeutic use*
  • Detergents
  • Disease Models, Animal
  • Glycodeoxycholic Acid
  • L-Lactate Dehydrogenase / metabolism
  • Lung / drug effects
  • Lung / metabolism
  • Lung / pathology
  • Male
  • Malondialdehyde / metabolism
  • Pancreas / drug effects
  • Pancreas / metabolism
  • Pancreas / microbiology
  • Pancreatitis, Acute Necrotizing / chemically induced
  • Pancreatitis, Acute Necrotizing / drug therapy*
  • Pancreatitis, Acute Necrotizing / metabolism
  • Peroxidase / metabolism
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / pharmacology
  • Phenylethyl Alcohol / therapeutic use
  • Rats
  • Rats, Sprague-Dawley


  • Antioxidants
  • Caffeic Acids
  • Detergents
  • Glycodeoxycholic Acid
  • Malondialdehyde
  • L-Lactate Dehydrogenase
  • Peroxidase
  • Alanine Transaminase
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol