Application of on-chip capillary electrophoresis to cell-free preparation of recombinant DNA

Nucleic Acids Symp Ser (Oxf). 2007:(51):87-8. doi: 10.1093/nass/nrm044.


An on-chip capillary electrophoresis-based DNA collection was applied to the isolation of target DNA species from a DNA mixture generated by a polymerase chain reaction (PCR), whose starting material was a ligation mixture of an insert and an expression vector. The collected DNA was then amplified by PCR and properly worked as template DNA in a coupled cell-free transcription/translation system. These results demonstrateed that total operation in standard genetic engineering can be performed in a cell-free condition.

MeSH terms

  • Cell-Free System
  • DNA, Recombinant / isolation & purification*
  • Electrophoresis, Microchip / instrumentation
  • Electrophoresis, Microchip / methods*
  • Polymerase Chain Reaction
  • Protein Biosynthesis
  • Templates, Genetic
  • Transcription, Genetic


  • DNA, Recombinant