Specific detection of PCR product from Legionella pneumophila strain Philadelphia1 using zinc finger protein Sp2

Kazunori Ikebukuro; Takenori Kumagai; Hiroaki Motoki; Yuko Osawa; Takafumi Matuo; Michio Horiuchi; Koji Sode

doi:10.1093/nass/nrm143

Specific detection of PCR product from Legionella pneumophila strain Philadelphia1 using zinc finger protein Sp2

Nucleic Acids Symp Ser (Oxf). 2007:(51):285-6. doi: 10.1093/nass/nrm143.

Authors

Kazunori Ikebukuro¹, Takenori Kumagai, Hiroaki Motoki, Yuko Osawa, Takafumi Matuo, Michio Horiuchi, Koji Sode

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture & Technology, 2-24-16 Naka-machi, Koganei, Tokyo 184-8588, Japan.

PMID: 18029698
DOI: 10.1093/nass/nrm143

Abstract

We have developed a novel method to detect PCR products from pathogen genome DNA by Zinc finger protein that can bind to double strand DNA (dsDNA) in sequence specific manner. In this study, we tried to detect Legionella pneumophila strain Philadelphia 1 using Zinc finger protein. We found the specific target DNA sequence for zinc finger protein Sp2 in L. pneumophila strain Philadelphia 1 genome DNA. Specific PCR product was successfully amplified from L. pneumophila strain Philadelphia 1 genome DNA and we used Zinc finger protein Sp2 to detect it. We succeeded in detecting the PCR products from L. pneumophila strain Philadelphia 1 genome DNA with Sp2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites
Legionella pneumophila / genetics
Legionella pneumophila / isolation & purification*
Molecular Sequence Data
Polymerase Chain Reaction
Sp2 Transcription Factor / chemistry
Sp2 Transcription Factor / metabolism*
Zinc Fingers

Substances

Sp2 Transcription Factor