Probing the mutation spectrum in E. coli

Nucleic Acids Symp Ser (Oxf). 2007;(51):289-90. doi: 10.1093/nass/nrm145.


The mutation spectrum, together with mutation frequency, is decisive for the size and nature of genetic diversity. We constructed plasmid-encoded probes for specific detection of each and all of the six base substitutions. Using the set of the probes, we analyzed the mutation spectrumon the plasmids caused by different types of mutagens and mutator enzymes/alleles.

MeSH terms

  • DNA Mutational Analysis / methods*
  • Escherichia coli / genetics*
  • Mutagenesis
  • Plasmids / genetics
  • beta-Lactamases / genetics


  • beta-Lactamases