Antisense activity of 2',4'-BNA targeted to PPAR gamma in THP-1 and HCT116 cells

Nucleic Acids Symp Ser (Oxf). 2007;(51):441-2. doi: 10.1093/nass/nrm221.

Abstract

2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) is a nucleic acid analogue that has high affinity binding to its complementary RNA. Peroxisome proliferator-activated receptor (PPAR) y belongs to the nuclear hormone receptor superfamily and is a drug target in the treatment of type 2 diabetes. Here, we show the antisense effects of 2',4'-BNA oligonucleotides against PPARy in the human monocytic leukaemia cell line THP-1 and the human colorectal tumor cell line HCT116.

MeSH terms

  • Bridged-Ring Compounds / chemistry
  • Bridged-Ring Compounds / pharmacology
  • Cell Line, Tumor
  • Gene Expression / drug effects
  • Humans
  • Hypoglycemic Agents / chemistry*
  • Hypoglycemic Agents / pharmacology*
  • Oligonucleotides, Antisense / chemistry
  • Oligonucleotides, Antisense / pharmacology*
  • PPAR gamma / genetics*
  • PPAR gamma / metabolism

Substances

  • Bridged-Ring Compounds
  • Hypoglycemic Agents
  • Oligonucleotides, Antisense
  • PPAR gamma