Identification in the mould Hypocrea jecorina of a gene encoding an NADP(+): d-xylose dehydrogenase

FEMS Microbiol Lett. 2007 Dec;277(2):249-53. doi: 10.1111/j.1574-6968.2007.00969.x.


A gene coding for an NADP(+)-dependent d-xylose dehydrogenase was identified in the mould Hypocrea jecorina (Trichoderma reesei). It was cloned from cDNA, the active enzyme was expressed in yeast and a histidine-tagged enzyme was purified and characterized. The enzyme had highest activity with d-xylose and significantly smaller activities with other aldose sugars. The enzyme is specific for NADP(+). The K(m) values for d-xylose and NADP(+) are 43 mM and 250 microM, respectively. The role of this enzyme in H. jecorina is unclear because in this organism d-xylose is predominantly catabolized through a path with xylitol and d-xylulose as intermediates and the mould is unable to grow on d-xylonic acid.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde Reductase / genetics*
  • Aldehyde Reductase / metabolism*
  • DNA, Complementary / isolation & purification
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism*
  • Gene Expression
  • Hypocrea / enzymology*
  • Hypocrea / genetics
  • Kinetics
  • Molecular Sequence Data
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Substrate Specificity
  • Xylose / metabolism


  • DNA, Complementary
  • Fungal Proteins
  • Recombinant Proteins
  • Xylose
  • Aldehyde Reductase
  • polyol dehydrogenase (NADP)

Associated data

  • GENBANK/EF136590