Rapid determination of the three-dimensional orientation of single molecules

Opt Lett. 2001 Feb 15;26(4):211-3. doi: 10.1364/ol.26.000211.

Abstract

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.