Determination of processing effects and of storage stability on vitamin K1 (Phylloquinone) in Sea Buckthorn Berries (Hippophaë rhamnoides L. ssp. rhamnoides) and related products

J Food Sci. 2007 Nov;72(9):C491-7. doi: 10.1111/j.1750-3841.2007.00567.x.


Phylloquinone (vitamin K(1)) is the primary dietary source of vitamin K. Processing effects and stability of phylloquinone were investigated during juice and concentrate production from sea buckthorn (Hippophaë rhamnoides) using berries from 2 different growing areas. During industrial juice production the technological processing of the berries caused a loss of about 36% to 54% phylloquinone in the generated juice. The following processing steps leading to the concentrated juice resulted in a complete depletion of phylloquinone. Sea buckthorn berries and juice were stored at 6, 25, and 40 degrees C for up to 7 d to determine the temperature effects on phylloquinone during storage. Content of vitamin K(1) in sea buckthorn berries was affected by storage time and storage temperature. Storage of freshly harvested berries resulted in a significant increase (P < 0.01) of phylloquinone ranging from 21% up to 186% (wet weight). The juices showed almost identical significant degradation (P < 0.01) of phylloquinone of about 18% to 32% at 6, 25, and 40 degrees C indicating that intensity of decomposition is independent of temperature (6 to 40 degrees C) and storage time in the range of consumer storage conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beverages / analysis*
  • Chromatography, High Pressure Liquid
  • Food Handling / methods*
  • Fruit / chemistry*
  • Fruit / metabolism
  • Hippophae*
  • Refrigeration
  • Reproducibility of Results
  • Temperature
  • Time Factors
  • Vitamin K 1 / analysis*
  • Vitamin K 1 / metabolism


  • Vitamin K 1