Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 6 (4), 267-77

Methyl Jasmonate Induced Apoptosis in Human Prostate Carcinoma Cells via 5-lipoxygenase Dependent Pathway

Affiliations
  • PMID: 18038760

Methyl Jasmonate Induced Apoptosis in Human Prostate Carcinoma Cells via 5-lipoxygenase Dependent Pathway

Daniel E Ezekwudo et al. J Exp Ther Oncol.

Abstract

Methyl jasmonate--a plant stress hormone with striking resemblance to lipoxygenase products have been reported to induce apoptosis in several cancers. However, 5-HETE--a product of the lipoxygenase pathway has been implicated in human prostate cancer progression and yet possible interaction between methyl jasmonate and the lipoxygenase pathway has not been reported, thus, leaving some unanswered questions on the mechanism(s) of action by methyl jasmonate. Using cytotoxicity and flow cytometry assays (BrdU assay) as well as fluorescence microscopy, we investigated the effects of the methyl jasmonate on the proliferation of human prostate adenocarcinoma cell lines (DU-145, PC-3) in vitro and the potential interaction between methyl jasmonate and the lipoxygenase pathway. Methyl jasmonate (MJ) significantly (p = 0.01) inhibited the proliferation of human prostate carcinoma cells in dose- and kinetic-dependent manners and showed specific interaction with 5-lipoxygenase (5-LOX) enzyme pathway. Flow cytometric analyses and fluorescence microscopy confirmed that the inhibition of proliferation was via the induction of apoptosis. Based on our findings, it can be proposed that the interaction of methyl jasmonate with 5-lipoxygenase pathway may participate in the observed anticarcinogenic property.

Similar articles

See all similar articles

Cited by 7 PubMed Central articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback