Time-gated biological imaging by use of colloidal quantum dots

Opt Lett. 2001 Jun 1;26(11):825-7. doi: 10.1364/ol.26.000825.


The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.