Identification of interleukin-1beta regulated genes in uterine smooth muscle cells

Reproduction. 2007 Dec;134(6):811-22. doi: 10.1530/REP-07-0289.


We analyzed the response of uterine smooth muscle cells to interleukin-1beta (IL-1beta). We first showed that PHM1-31 myometrial cells, our cellular model, are contractile. To determine the molecular mechanisms of uterine smooth muscle cell activation by proinflammatory cytokines, we performed genechip expression array profiling studies of PHM1-31 cells in the absence and the presence of IL-1beta. In total, we identified 198 known genes whose mRNA levels are significantly modulated (> 2.0-fold change) following IL-1beta exposure. We confirmed the expression changes for selected genes by independent mRNA and protein analysis. The group of genes induced by IL-1beta includes transcription factors and inflammatory response genes such as nuclear factor of kappa light polypeptide gene enhancer in B-cells (NFkappaB), pentraxin-related gene (PTX3), and tumor necrosis factor alpha-induced protein 3/A20 (TNFAIP3/A20). We also found up-regulation of chemokines like C-X-C motif ligand 3 (CXCL3) and extracellular matrix remodeling signaling molecules like tenascin C (TNC). Our data suggest that IL-1beta elicits the rapid activation of a cellular network of genes particularly implicated in inflammatory response that may create a cellular environment favorable for myometrial cell contraction. Our results provide novel insights into the mechanisms of uterine smooth muscle cell regulation and possibly infection-induced preterm labor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern / methods
  • Cell Line
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation / drug effects*
  • Humans
  • Immunoblotting / methods
  • Interleukin-1beta / pharmacology*
  • Myocardial Contraction
  • Myocytes, Smooth Muscle / drug effects
  • Myocytes, Smooth Muscle / immunology*
  • Myometrium / drug effects
  • Myometrium / immunology*
  • Obstetric Labor, Premature / immunology*
  • Oligonucleotide Array Sequence Analysis*
  • Pregnancy
  • Stimulation, Chemical


  • Interleukin-1beta