Rhodanobacter ginsengisoli sp. nov. and Rhodanobacter terrae sp. nov., isolated from soil cultivated with Korean ginseng

Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2810-2813. doi: 10.1099/ijs.0.65018-0.

Abstract

Two bacterial isolates from ginseng fields in Korea, strains GR17-7(T) and GP18-1(T), were characterized using a polyphasic approach. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the Gammaproteobacteria, and showed that the closest phylogenetic relationships were with members of the genus Rhodanobacter. The 16S rRNA gene sequence similarity between strains GR17-7(T) and GP18-1(T) was 97.2 %. Both strains showed 16S rRNA gene sequence similarities of 95.2-96.9 % to type strains of recognized Rhodanobacter species. The G+C contents of the DNA of strains GR17-7(T) and GP18-1(T) were 61.0 and 62.5 mol%, respectively. According to the DNA-DNA hydridization tests, the hybridization value between strains GR17-7(T) and GP18-1(T) was 34 %. Strains GR17-7(T) and GP18-1(T) showed less than 32 % DNA-DNA relatedness with Rhodanobacter fulvus KCTC 12098(T) and Rhodanobacter spathiphylli LMG 23181(T). Strains GR17-7(T) and GP18-1(T) were aerobic, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Major fatty acids of both strains were iso-C(17 : 1)omega9c and iso-C(16 : 0). Based on the data presented, two novel Rhodanobacter species are proposed, with the names Rhodanobacter ginsengisoli sp. nov. (type strain GR17-7(T)=KACC 11762(T)=DSM 18993(T)) and Rhodanobacter terrae sp. nov. (type strain GP18-1(T)=KACC 11761(T)=DSM 19241(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Catalase / analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Korea
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oxidoreductases / analysis
  • Panax
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Soil Microbiology*
  • Xanthomonadaceae / chemistry
  • Xanthomonadaceae / classification*
  • Xanthomonadaceae / genetics
  • Xanthomonadaceae / isolation & purification*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Oxidoreductases
  • Catalase

Associated data

  • GENBANK/EF166075
  • GENBANK/EF166076