Crystallographic investigation of the inhibition mode of a VIM-2 metallo-beta-lactamase from Pseudomonas aeruginosa by a mercaptocarboxylate inhibitor

J Med Chem. 2007 Dec 27;50(26):6647-53. doi: 10.1021/jm701031n. Epub 2007 Dec 6.

Abstract

The VIM-2 metallo-beta-lactamase enzyme from Pseudomonas aeruginosa catalyzes the hydrolysis of most beta-lactam antibiotics including carbapenems, and there are currently no potent inhibitors of such enzymes. We found rac-2-omega-phenylpropyl-3-mercaptopropionic acid, phenylC3SH, to be a potent inhibitor of VIM-2. The structure of the VIM-2-phenylC3SH complex was determined by X-ray crystallography to 2.3 A. The structure revealed that the thiol group of phenylC3SH bridged to the two zinc(II) ions and the phenyl group interacted with Tyr67(47) on loop1 near the active site, by pi-pi stacking interactions. The methylene group interacted with Phe61(42) located at the bottom of loop1 through CH-pi interactions. Dynamic movements were observed in Arg228(185) and Asn233(190) on loop2, compared with the native structure (PDB code: 1KO3 ). These results suggest that the above-mentioned four residues play important roles in the binding and recognition of inhibitors or substrates and in stabilizing a loop in the VIM-2 enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Mercaptopropionic Acid / analogs & derivatives*
  • 3-Mercaptopropionic Acid / chemistry
  • Binding Sites
  • Crystallography, X-Ray
  • Models, Molecular
  • Protein Binding
  • Protein Conformation
  • Pseudomonas aeruginosa / enzymology*
  • beta-Lactamase Inhibitors*
  • beta-Lactamases / chemistry*

Substances

  • 2-omega-phenylpropyl-3-mercaptopropionic acid
  • beta-Lactamase Inhibitors
  • 3-Mercaptopropionic Acid
  • VIM-2 beta-lactamase
  • beta-Lactamases

Associated data

  • PDB/1KO3