The effect of moderate ethanol doses (0.5 and 1 g/kg body weight) on serum prolactin (PRL), cortisol, epinephrine and norepinephrine concentrations was measured in a double-blind, placebo-controlled study. Eight healthy male volunteers participated. Blood was with drawn twice before, and 30 min, 60 min, 90 min and 120 min after oral ethanol intake. The higher ethanol dose, but not the lower dose, significantly increased PRL concentrations. Concentrations of cortisol, epinephrine and norepinephrine were not changed by either dose. Thus, the effect of ethanol on PRL appears to be dose-dependent and not stress-related.