Expression profile of ErbB receptor's family in human alveolar type 2-like cell line A549 exposed to hexavalent chromium

Toxicol In Vitro. 2008 Mar;22(2):541-7. doi: 10.1016/j.tiv.2007.10.003. Epub 2007 Oct 14.


Occupational exposure to hexavalent chromium (Cr (VI)) compounds is associated with increased risk of pulmonary disease. In the present study we have investigated temporal expression of ErbB's receptors family in A549 cells after exposure to Cr (VI). Treatment with 10 microM or 300 microM of Na2CrO4 induced apoptotic cell death within 24h. Based on data obtained by ELISA cell death detection method and fluorescence microscopy, the concentration of 10 microM was chosen to study the expression of ErbB receptors family. Such concentration reflects a condition of acute toxicity in which cells survived up to 24h. Real time quantitative PCR has been performed to analyze the expression profiles of ErbB family genes following chromium toxicity. The expression of EGFR and ErbB2 receptors was significantly reduced after 1h and 4h of treatment while ErbB2 receptor was significantly increased and EGFR receptor returned to basal value after 24h. Instead, ErbB3 receptor was overexpressed after 1h, returned to basal level after 4h and increased its level after 24h. Exposure to chromium did not change expression level of ErbB4 receptor in A549 cell line. The present data suggests that expression changes in ErbB receptors might have a role in the carcinogenic effects induced by this pneumotoxic agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Chromium / toxicity*
  • ErbB Receptors / biosynthesis
  • Gene Expression / drug effects
  • Genes, erbB / genetics*
  • Humans
  • Lung Neoplasms / pathology
  • Microscopy, Fluorescence
  • Nucleosomes / drug effects
  • Pulmonary Alveoli / cytology
  • Pulmonary Alveoli / drug effects
  • Pulmonary Alveoli / metabolism*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction


  • Nucleosomes
  • RNA, Messenger
  • Chromium
  • chromium hexavalent ion
  • ErbB Receptors