Voxel-based analysis of R2* maps in the healthy human brain

J Magn Reson Imaging. 2007 Dec;26(6):1413-20. doi: 10.1002/jmri.21204.


Purpose: To develop a voxel-based analysis of an R2* map of healthy human brain that is automatic, reproducible, and realizable in a single examination on a 3T MR imager. Such a tool could be useful to measure iron accumulation in neurodegenerative diseases.

Materials and methods: In all, 18 healthy subjects underwent MR imaging at a field strength of 3T: 1) six consecutive T2*-weighted gradient-echo volumes were acquired using a segmented echo-planar imaging sequence and 2) a conventional dual-echo turbo spin echo sequence was also applied to acquire T2-weighted images. Images were realigned and spatial correction was performed using a template brain dataset with SPM2. For each subject we performed a voxel-by-voxel nonlinear least-squares fitting of the data acquired at the six echo times to obtain a monoexponential signal decay curve. The reproducibility and sensitivity to age variation were assessed by voxel-based analysis.

Results: The reproducibility tests in whole brain analysis showed little R2* variation. Furthermore, the statistical analysis, performed on each brain voxel, revealed a significant positive correlation between age and MR values located in regions where a slow and constant age-related iron deposition is known.

Conclusion: Our method, combining data acquisition and data processing, demonstrates the feasibility of voxel-based analysis on an R2* map and affords a high degree of sensitivity and good reproducibility while maintaining high spatial resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Artifacts
  • Brain Mapping / methods*
  • Female
  • Humans
  • Image Enhancement / methods*
  • Image Processing, Computer-Assisted / methods*
  • Least-Squares Analysis
  • Magnetic Resonance Imaging / methods*
  • Male
  • Reproducibility of Results
  • Sensitivity and Specificity