Immunoreactivity for CD25 in gastrointestinal mucosal mast cells is specific for systemic mastocytosis

Am J Surg Pathol. 2007 Nov;31(11):1669-76. doi: 10.1097/PAS.0b013e318078ce7a.


Systemic mastocytosis (SM) is characterized by the accumulation of neoplastic mast cells in bone marrow and other organs. Gastrointestinal (GI) symptoms are common in both SM and cutaneous mastocytosis [urticaria pigmentosa (UP)], and are usually caused by the release of histamine and other inflammatory mediators. Occasionally, neoplastic mast cells may also directly infiltrate the GI tract. Previous studies have suggested that enumeration of the mast cells in GI biopsies may help establish the diagnosis of SM. However, mast cells have been reported to be increased in various inflammatory diseases, and mast cell density has not been systematically evaluated in other GI disorders. Recently, expression of CD25 by mast cells in bone marrow has been shown to be specific for SM. The purpose of this study was (1) to quantitate and compare mast cells in mucosal biopsies from patients with SM involving the GI tract, UP with GI symptoms, and a control group of diverse inflammatory disorders, and (2) to determine whether immunostaining for CD25 can be used to distinguish neoplastic from reactive mast cells in GI biopsies. Seventeen GI biopsies from 6 patients with SM; 17 GI biopsies from 5 patients with UP; and 157 control cases including 10 each normal stomach, duodenum, terminal ileum, and colon, Helicobacter pylori gastritis, bile reflux gastropathy, peptic duodenitis, celiac disease, Crohn disease, ulcerative colitis, lymphocytic colitis, and collagenous colitis, 20 biopsies from 16 patients with irritable bowel syndrome, 8 biopsies from 5 patients with parasitic infections, and 9 biopsies from 7 patients with eosinophilic gastroenteritis were immunostained for mast cell tryptase, c-kit (CD117), and CD25. Mucosal mast cells were quantitated, and the presence or absence of CD25 expression on mast cells was determined. In SM patients, mast cells in the small intestine and colon numbered >100/high-power field (HPF) in nearly all cases (mean 196/HPF; range 74 to 339). This was significantly higher than in GI biopsies from UP patients (mean 17/HPF; range 8 to 32, P<0.0001) and all inflammatory diseases (P<0.01). Mast cell density in other disorders ranged from a mean of 12/HPF in H. pylori gastritis to 47/HPF in parasitic infections. Interestingly, all SM biopsies (and none of the other cases) contained aggregates or confluent sheets of mast cells. In addition, mast cells in all SM cases were positive for CD25, whereas GI mucosal mast cells in UP and all other control cases were negative. In conclusion, quantitation of mast cells can be helpful to diagnose SM in GI mucosal biopsies, although mast cells are also markedly increased in parasitic infections. Aggregates or sheets of mast cells are only seen in SM. Immunoreactivity for CD25 in GI mucosal mast cells is specific for SM and can be used to confirm the diagnosis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Gastrointestinal Diseases / diagnosis*
  • Gastrointestinal Diseases / immunology
  • Gastrointestinal Diseases / pathology
  • Humans
  • Immunohistochemistry
  • Interleukin-2 Receptor alpha Subunit / analysis*
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / pathology
  • Intestines / immunology*
  • Intestines / pathology
  • Mast Cells / immunology*
  • Mast Cells / pathology
  • Mastocytosis, Systemic / diagnosis*
  • Mastocytosis, Systemic / immunology
  • Mastocytosis, Systemic / pathology
  • Proto-Oncogene Proteins c-kit / analysis
  • Urticaria Pigmentosa / diagnosis*
  • Urticaria Pigmentosa / immunology
  • Urticaria Pigmentosa / pathology


  • Interleukin-2 Receptor alpha Subunit
  • Proto-Oncogene Proteins c-kit