Abstract
In tumor cells, alterations in cellular glycosylation may play a key role in their metastatic behavior. Using small interfering RNA against GnT-V, we found that the expression of GnT-V and beta1,6GlcNAc branching were significantly reduced which was particularly accompanied by the arrest in both cell migration and invasion as compared to the negative control. Moreover, the suppressed GnT-V expression by siRNA technique inactivated the signaling molecules including Rac1, cofilin, Erk and Akt, and activated RhoA levels in cells lacking GnT-V, but revealed no impact on Cdc42 activity. All these notions disclose for the first time that GnT-V and beta1, 6GlcNAc branching mediate the cell migration and invasion in Rac1-positive and RhoA-negative regulatory manners.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / metabolism
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Actin Depolymerizing Factors / metabolism
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Actins / metabolism
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Animals
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Cell Adhesion / drug effects
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Cell Line, Tumor
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Cell Movement* / drug effects
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Cytoskeleton / drug effects
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Cytoskeleton / metabolism
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Down-Regulation / drug effects
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Down-Regulation / genetics
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Enzyme Activation / drug effects
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Female
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Fibronectins / metabolism
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Mammary Neoplasms, Animal / enzymology*
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Mammary Neoplasms, Animal / pathology*
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Mice
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N-Acetylglucosaminyltransferases / genetics
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N-Acetylglucosaminyltransferases / metabolism*
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Neoplasm Invasiveness
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Phosphorylation / drug effects
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Proto-Oncogene Proteins c-akt / metabolism
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RNA, Small Interfering / pharmacology
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Signal Transduction* / drug effects
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cdc42 GTP-Binding Protein / metabolism
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rac1 GTP-Binding Protein / metabolism*
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rhoA GTP-Binding Protein / metabolism*
Substances
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Actin Depolymerizing Factors
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Actins
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Fibronectins
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RNA, Small Interfering
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N-Acetylglucosaminyltransferases
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alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
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Proto-Oncogene Proteins c-akt
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Extracellular Signal-Regulated MAP Kinases
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cdc42 GTP-Binding Protein
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rac1 GTP-Binding Protein
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rhoA GTP-Binding Protein
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Acetylglucosamine