A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling

Bioorg Med Chem Lett. 2008 Jan 15;18(2):650-2. doi: 10.1016/j.bmcl.2007.11.071. Epub 2007 Nov 22.

Abstract

A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl)phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin / chemistry*
  • Ceramides / chemistry*
  • Chromatography, Thin Layer
  • Electrophoresis, Polyacrylamide Gel
  • Luminescence
  • Photoaffinity Labels*

Substances

  • Ceramides
  • Photoaffinity Labels
  • Biotin