DNA damage induces two distinct modes of cell death in ovarian carcinomas

Cell Death Differ. 2008 Mar;15(3):555-66. doi: 10.1038/sj.cdd.4402286. Epub 2007 Dec 7.

Abstract

Activation of p53 by cellular stress may lead to either cell cycle arrest or apoptotic cell death. Restrictions in a cell's ability to halt the cell cycle might, in turn, cause mitotic catastrophe, a delayed type of cell death with distinct morphological features. Here, we have investigated the contribution of p53 and caspase-2 to apoptotic cell death and mitotic catastrophe in cisplatin-treated ovarian carcinoma cell lines. We report that both functional p53 and caspase-2 were required for the apoptotic response, which was preceded by translocation of nuclear caspase-2 to the cytoplasm. In the absence of functional p53, cisplatin treatment resulted in caspase-2-independent mitotic catastrophe followed by necrosis. In these cells, apoptotic functions could be restored by transient expression of wt p53. Hence, p53 appeared to act as a switch between apoptosis and mitotic catastrophe followed by necrosis-like lysis in this experimental model. Further, we show that inhibition of Chk2, and/or 14-3-3sigma deficiency, sensitized cells to undergo mitotic catastrophe upon treatment with DNA-damaging agents. However, apoptotic cell death seemed to be the final outcome of this process. Thus, we hypothesize that the final mode of cell death triggered by DNA damage in ovarian carcinoma cells is determined by the profile of proteins involved in the regulation of the cell cycle, such as p53- and Chk2-related proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Antineoplastic Agents / toxicity*
  • Apoptosis*
  • Carcinoma / enzymology
  • Carcinoma / metabolism
  • Carcinoma / pathology*
  • Caspase 2 / metabolism
  • Cell Line, Tumor
  • Cell Nucleus / enzymology
  • Checkpoint Kinase 2
  • Cisplatin / toxicity*
  • DNA Damage*
  • Female
  • Humans
  • Mitosis
  • Necrosis*
  • Ovarian Neoplasms / enzymology
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology*
  • Protein-Serine-Threonine Kinases / antagonists & inhibitors
  • Tumor Suppressor Protein p53 / antagonists & inhibitors
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Antineoplastic Agents
  • Tumor Suppressor Protein p53
  • Checkpoint Kinase 2
  • CHEK2 protein, human
  • Protein-Serine-Threonine Kinases
  • Caspase 2
  • Cisplatin