Antioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation

Biol Res. 2007;40(2):155-62. doi: 10.4067/s0716-97602007000200006. Epub 2007 Nov 21.

Abstract

The antioxidant effect of 5-Aminosalicylic acid (5-ASA) on copper-mediated LDL oxidation was followed either by the emitted chemiluminiscence (CL) or by UV-vis spectroscopy. 5-ASA addition extends the lag phase in a concentration-dependent manner without changes in the rate of the process in the autoaccelerated phase. The antioxidant behavior of 5-ASA was very similar to that of Trolox, a very efficient water soluble antioxidant. The copper-binding capacity of 5-ASA was evaluated by UV-visible spectroscopy. The addition of copper to a 5-ASA solution increases the absorbance at 332 nm and generates a new band at 298 nm. These changes in the UV-vis spectra indicate formation of a complex between 5-ASA and copper. However, LDL protection by 5-ASA is unrelated to its copper chelating capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminosalicylic Acids / chemistry
  • Aminosalicylic Acids / metabolism
  • Aminosalicylic Acids / pharmacology*
  • Antioxidants / chemistry
  • Antioxidants / metabolism
  • Antioxidants / pharmacology*
  • Copper / chemistry*
  • Copper / toxicity
  • Lipoproteins, LDL / metabolism*
  • Luminescent Measurements
  • Oxidation-Reduction / drug effects
  • Spectrophotometry, Ultraviolet
  • Time Factors

Substances

  • Aminosalicylic Acids
  • Antioxidants
  • Lipoproteins, LDL
  • Copper