RNAi activity of siRNAs modified with 2'-aminoalkyl-substituted fluorinated nucleobases

Nucleosides Nucleotides Nucleic Acids. 2007;26(6-7):865-8. doi: 10.1080/15257770701504033.


We recently reported that a 1'-deoxy-1'-(4,6-difluoro-1H-benzimidazol-1-yl)-2'-(beta-aminoethyl)-beta-d-ribofuranose nucleoside appears to be a universal nucleoside which does not differentiate between the four natural nucleosides A, C, G, and U in duplexes. Moreover, ribozymes modified with this nucleoside analog showed a better or at least equal catalytic activity relative to Watson-Crick mismatches.[1] Due to these data, we investigated the ability of this compound to tolerate Watson-Crick mismatches in order to avoid HIV escape mutations in RNA interference. The influence of this nucleoside analog on siRNA efficiency was analyzed with a proven siRNA targeting GFP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Fluorine Compounds / chemistry*
  • Gene Expression Regulation
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Nucleosides / chemistry*
  • RNA Interference*
  • RNA, Messenger
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism*


  • Fluorine Compounds
  • Nucleosides
  • RNA, Messenger
  • RNA, Small Interfering
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins