Combined effects of vitamin C, vitamin E, and sodium selenate supplementation on absolute ethanol-induced injury in various organs of rats

Int J Toxicol. 2007 Nov-Dec;26(6):513-23. doi: 10.1080/10915810701707296.


In this study, the effect of combination of vitamin C (ascorbic acid), vitamin E (alpha -tocopherol), and selenium (sodium selenate) on ethanol-induced liver and intestine injury in rats was investigated. The ethanol-induced injury was produced by the administration of 1 ml of absolute ethanol to each rats. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg), and sodium selenate (Se) (0.5 mg/kg) for 3 days; 1 h after the final antioxidant administration, they were sacrificed. Lipid peroxidation and glutathione levels, catalase (CAT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GP(x)) activities were determined in liver and intestine tissues. Myeloperoxidase (MPO), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) were determined in liver tissue. Also, CAT activity, urea, creatinine, uric acid, and total lipid levels were determined in serum samples. In the ethanol group, serum urea, creatinine, uric acid, and total lipid levels; liver and intestine LDH; liver MPO, AST, ALP, ALT, and GGT activities; and liver and intestine LPO levels increased, whereas serum CAT activity, liver and intestine GSH levels, and CAT, SOD, and GP(x) activities decreased. On the other hand, treatment with vitamin C, vitamin E, and Se reversed these effects. As a result of these findings, we can say that the combination of vitamin C, vitamin E, and selenium has a protective effect on ethanol-induced changes in lipid peroxidation, glutathione levels, and antioxidant enzyme activities in liver and intestine tissues, and in some serum parameters of rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology*
  • Catalase / blood
  • Creatinine / blood
  • Duodenum / drug effects
  • Duodenum / metabolism
  • Ethanol / toxicity*
  • Female
  • Glutathione / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Oxidoreductases / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Selenic Acid
  • Selenium Compounds / pharmacology*
  • Transferases / metabolism
  • Urea / blood
  • Uric Acid / blood
  • Vitamin E / pharmacology*


  • Antioxidants
  • Selenium Compounds
  • Vitamin E
  • Uric Acid
  • Ethanol
  • Urea
  • Creatinine
  • Oxidoreductases
  • Catalase
  • Transferases
  • Alkaline Phosphatase
  • Glutathione
  • Selenic Acid
  • Ascorbic Acid