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, 366 (2), 414-9

Biotransformation of (+)-Catechin Into Taxifolin by a Two-Step Oxidation: Primary Stage of (+)-Catechin Metabolism by a Novel (+)-Catechin-Degrading Bacteria, Burkholderia Sp. KTC-1, Isolated From Tropical Peat

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Biotransformation of (+)-Catechin Into Taxifolin by a Two-Step Oxidation: Primary Stage of (+)-Catechin Metabolism by a Novel (+)-Catechin-Degrading Bacteria, Burkholderia Sp. KTC-1, Isolated From Tropical Peat

Motoki Matsuda et al. Biochem Biophys Res Commun.

Abstract

Peat contains various persistent compounds derived from plant materials. We isolated a novel (+)-catechin-degrading bacterium, Burkholderia sp. KTC-1 (KTC-1), as an example of a bacterium capable of degrading persistent aromatic compounds present in tropical peat. This bacterium was isolated by an enrichment technique and grew on (+)-catechin as the sole carbon source under acidic conditions. The reaction of a crude enzyme extract and a structural study of its products showed that (+)-catechin is biotransformed into taxifolin during the preliminary stages of its metabolism by KTC-1. HPLC analysis showed that this transformation occurs in two oxidation steps: 4-hydroxylation and dehydrogenation. Furthermore, both (+)-catechin 4-hydroxylanase and leucocyanidin 4-dehydrogenase were localized in the cytosol of KTC-1. This is the first report on biotransformation of (+)-catechin into taxifolin via leucocyanidin by an aerobic bacterium. We suggest that tropical peat could become a unique resource for microorganisms that degrade natural aromatic compounds.

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