The nucleoporin Nup358 associates with and regulates interphase microtubules

FEBS Lett. 2008 Jan 23;582(2):190-6. doi: 10.1016/j.febslet.2007.11.087. Epub 2007 Dec 10.


The nucleoporin Nup358 resides on the cytoplasmic face of the interphase nuclear pore complex (NPC). During metaphase, its recruitment to kinetochores is important for correct microtubule-kinetochore attachment. Here, we report that a fraction of endogenous Nup358 interacts with interphase microtubules through its N-terminal region (BPN). Cells overexpressing the microtubule targeting domain of Nup358 displayed dramatic alteration in the microtubule organization including increased microtubule bundling and stability. Ectopic expression of BPN and full-length Nup358 exhibited significantly higher levels of acetylated microtubules that were resistant to nocodazole, a microtubule depolymerizing agent. Furthermore, RNAi mediated depletion of Nup358 affected polarized stabilization of microtubules during directed cell migration, confirming the in vivo role of Nup358 in regulating interphase microtubules.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Base Sequence
  • CHO Cells
  • COS Cells
  • Chlorocebus aethiops
  • Cricetinae
  • Cricetulus
  • DNA Primers
  • Interphase*
  • Microtubules / physiology*
  • Molecular Chaperones / physiology*
  • Nuclear Pore Complex Proteins / physiology*
  • RNA Interference


  • DNA Primers
  • Molecular Chaperones
  • Nuclear Pore Complex Proteins
  • ran-binding protein 2