Quantitative Telomeric Overhang Determination Using a Double-Strand Specific Nuclease

Nucleic Acids Res. 2008 Feb;36(3):e14. doi: 10.1093/nar/gkm1063. Epub 2007 Dec 10.

Abstract

Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.

Publication types

  • Research Support, N.I.H., Extramural
  • Validation Study

MeSH terms

  • Blotting, Southern*
  • DNA / metabolism
  • Endodeoxyribonucleases / metabolism*
  • HeLa Cells
  • Humans
  • Telomere / chemistry*
  • Telomere / metabolism

Substances

  • DNA
  • Endodeoxyribonucleases