Objective: To investigate the method of determination of total alkaloids Uncaria rhynchophylla.
Methods: The Contents of total Alkaloid were determined by Acid dye Colorimetry.
Results: Acid dye color conditions: pH3.6 buffer 5.0 ml, bromocresol green liquid 5.0 ml; chloroform extraction three times, each time was exeracted for 2 minutes, put it aside for at least 5 minutes for the determination of the best method. Rhynchophylline 6.018 microg - 108.324 microg in the linear range, Recoveriys rate was 97.19%, RSD was 1.34% (n = 6).
Conclusion: The method is simple, highly sensitive and reproducible.