Human Parechovirus Type 1, 3, 4, 5, and 6 Detection in Picornavirus Cultures

J Clin Microbiol. 2008 Feb;46(2):759-62. doi: 10.1128/JCM.02009-07. Epub 2007 Dec 12.

Abstract

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Humans
  • Molecular Sequence Data
  • Neutralization Tests
  • Parechovirus / classification
  • Parechovirus / genetics*
  • Parechovirus / isolation & purification*
  • Picornaviridae / growth & development*
  • Picornaviridae Infections / diagnosis
  • Picornaviridae Infections / virology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Virus Cultivation*

Substances

  • Viral Proteins

Associated data

  • GENBANK/EF155422
  • GENBANK/EF155423
  • GENBANK/EU077500
  • GENBANK/EU077501
  • GENBANK/EU077502
  • GENBANK/EU077503
  • GENBANK/EU077504
  • GENBANK/EU077505
  • GENBANK/EU077506
  • GENBANK/EU077507
  • GENBANK/EU077508
  • GENBANK/EU077509
  • GENBANK/EU077514
  • GENBANK/EU077515
  • GENBANK/EU077516
  • GENBANK/EU077517
  • GENBANK/EU077518
  • GENBANK/EU077519
  • GENBANK/EU077520
  • GENBANK/EU077521
  • GENBANK/EU077522
  • GENBANK/EU077523
  • GENBANK/EU077524