Requirement for CD1d expression by B cells to stimulate NKT cell-enhanced antibody production

Blood. 2008 Feb 15;111(4):2158-62. doi: 10.1182/blood-2007-10-117309. Epub 2007 Dec 12.


Activation of natural killer-like T (NKT) cells with the CD1d ligand alpha-galactosylceramide enhances T-dependent humoral immune responses against coadministered T-dependent Ag. At present, there is little information on the mechanisms involved other than a dependence on CD1d expression by antigen-presenting cells and/or development of the NKT subset. We therefore tested the hypothesis that direct presentation of alpha-GC by B cells was required for NKT-enhanced Ab responses against T-dependent Ag. We reconstituted B cell-deficient microMT mice with B cells from C57Bl/6 donors or CD1d(-/-) donors before immunization with NP-KLH alone or NP-KLH mixed with alpha-GC. We made the surprising observation that B-cell expression of CD1d is absolutely required for the NKT-enhanced Ab response. Our data show that the mechanism by which NKT cells enhance humoral immune responses involves interaction with CD1d-expressing B cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antibody Formation*
  • Antigens, CD / immunology
  • Antigens, CD1 / genetics
  • Antigens, CD1 / immunology*
  • Antigens, CD1d
  • B-Lymphocytes / immunology*
  • Galactosylceramides / pharmacology
  • Humans
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology*
  • Lymphocyte Activation / drug effects
  • Mice
  • Mice, Knockout
  • Spleen / immunology
  • Thymus Gland / immunology


  • Antigens, CD
  • Antigens, CD1
  • Antigens, CD1d
  • CD1D protein, human
  • Galactosylceramides
  • alpha-galactosylceramide