Interleukin-4 stimulates collagen synthesis by normal and scleroderma fibroblasts in dermal equivalents

Cell Mol Biol. 1991;37(8):823-9.


Interleukin-4 (IL-4) is one of the products of T-lymphocytes and mast cells, inflammatory cells which accumulate in connective tissues at early stages of fibrosis. We tested the effects of IL-4 on human fibroblasts from normal and scleroderma skin seeded in three dimensional collagen lattices ("dermal equivalents"). IL-4 (10 and 100 U/ml) stimulated collagen synthesis in a dose-dependent manner. No significant alteration of lattice retraction and cell proliferation was observed. At the concentration 100 U/ml, Il-4 was approximately twice more efficient on collagen synthesis than Transforming Growth Factor beta (10 ng/ml). IL-4 secretion in connective tissues might be an important factor for the development of fibrotic processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Collagen / metabolism*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism*
  • Humans
  • Interleukin-4 / pharmacology*
  • Proteins / metabolism
  • Scleroderma, Localized / metabolism*
  • Skin / drug effects
  • Skin / metabolism*


  • Proteins
  • Interleukin-4
  • Collagen