A method making use of turbulent flow chromatography automated online extraction with tandem mass spectrometry (MS/MS) was developed for the analysis of 4 quinolones and 12 fluoroquinolones in honey. The manual sample preparation was limited to a simple dilution of the honey test portion in water followed by a filtration. The extract was online purified on a large particle size extraction column where the sample matrix was washed away while the analytes were retained. Subsequently, the analytes were eluted from the extraction column onto an analytical column by means of an organic solvent prior to chromatographic separation and MS detection. Validation was performed at three fortification levels (i.e., 5, 20, and 50 microg/kg) in three different honeys (acacia, multiflower, and forest) using the single-point calibration procedure by means of either a 10 or 25 microg/kg calibrant. Good recovery (85-127%, median 101%) as well as within-day (2-18%, median 6%) and between-day (2-42%, median 9%) precision values was obtained whatever the level of fortification and the analyte surveyed. Due to the complexity of the honey matrix and the large variation of the MS/MS transition reaction signals, which were honey-dependent, the limit of quantification for all compounds was arbitrarily set at the lowest fortification level considered during the validation, e.g., 5 microg/kg. This method has been successfully applied in a minisurvey of 34 honeys, showing ciprofloxacin and norfloxacin as the main (fluoro)quinolone antibiotics administered to treat bacterial diseases of bees. Turbulent flow chromatography coupled to LC-MS/MS showed a strong potential as an alternative method compared to those making use of offline sample preparation, in terms of both increasing the analysis throughput and obtaining higher reproducibility linked to automation to ensure the absence of contaminants in honey samples.