A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea

Science. 2007 Dec 14;318(5857):1782-6. doi: 10.1126/science.1149976.

Abstract

The assimilation of carbon dioxide (CO2) into organic material is quantitatively the most important biosynthetic process. We discovered that an autotrophic member of the archaeal order Sulfolobales, Metallosphaera sedula, fixed CO2 with acetyl-coenzyme A (acetyl-CoA)/propionyl-CoA carboxylase as the key carboxylating enzyme. In this system, one acetyl-CoA and two bicarbonate molecules were reductively converted via 3-hydroxypropionate to succinyl-CoA. This intermediate was reduced to 4-hydroxybutyrate and converted into two acetyl-CoA molecules via 4-hydroxybutyryl-CoA dehydratase. The key genes of this pathway were found not only in Metallosphaera but also in Sulfolobus, Archaeoglobus, and Cenarchaeum species. Moreover, the Global Ocean Sampling database contains half as many 4-hydroxybutyryl-CoA dehydratase sequences as compared with those found for another key photosynthetic CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase-oxygenase. This indicates the importance of this enzyme in global carbon cycling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl Coenzyme A / metabolism
  • Acetyl-CoA Carboxylase / metabolism
  • Acyl Coenzyme A / metabolism
  • Amino Acid Sequence
  • Anaerobiosis
  • Archaea / genetics
  • Archaea / metabolism
  • Autotrophic Processes
  • Bicarbonates / metabolism
  • Carbon Dioxide / metabolism*
  • Genes, Archaeal
  • Hydro-Lyases / genetics
  • Hydro-Lyases / metabolism
  • Hydroxybutyrates / metabolism*
  • Kinetics
  • Lactic Acid / analogs & derivatives*
  • Lactic Acid / metabolism
  • Metabolic Networks and Pathways
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Photosynthesis
  • Phylogeny
  • Sulfolobaceae / genetics
  • Sulfolobaceae / metabolism*

Substances

  • Acyl Coenzyme A
  • Bicarbonates
  • Hydroxybutyrates
  • Carbon Dioxide
  • 4-hydroxybutyric acid
  • Lactic Acid
  • succinyl-coenzyme A
  • Acetyl Coenzyme A
  • hydracrylic acid
  • 4-hydroxybutyryl-CoA dehydratase
  • Hydro-Lyases
  • Acetyl-CoA Carboxylase