Physical properties of two types of calcium stores and SERCAs in human platelets

Mol Cell Biochem. 2008 Apr;311(1-2):9-18. doi: 10.1007/s11010-007-9687-z. Epub 2007 Dec 16.


The aim of this work was to obtain experimental data depending on the properties of calcium stores and SERCAs, to analyse these data in terms of the models based on simulation of the cellular compartments as communicating vessels, and to relate this way the data to the above properties. The main characteristics of the stores and corresponding SERCAs have been estimated. Calcium content in the DTS is approximately 1.5 x 10(6) ions per cell, that in the acidic stores, approximately 0.64 x 10(6) ions per cell. The rate constant of background calcium leakage from the DTS is approximately 0.0033 s(-1), that from the acidic stores, approximately 0.1 s(-1). The background activity of SERCA2b is approximately 0.22 x 10(6 )s(-1) ions per cell, that of SERCA3, approximately 2.5 x 10(6 )s(-1) ions per cell. The properties of both calcium stores and the SERCAs and the characteristics found might be related to physiological or pathological state of the cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets / metabolism*
  • Calcium / metabolism*
  • Chelating Agents / metabolism
  • Fluorescent Dyes / metabolism
  • Fura-2 / metabolism
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism*
  • Models, Biological
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / genetics
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism*


  • Chelating Agents
  • Fluorescent Dyes
  • Isoenzymes
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Calcium
  • Fura-2