Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues : cDNA sequence and expression of tree shrew ABCA1

Mol Biol Rep. 2009 Mar;36(3):443-8. doi: 10.1007/s11033-007-9199-7. Epub 2007 Dec 16.

Abstract

ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was identified using SMART-RACE and Real-Time PCR techniques respectively. The nucleotide sequence of tree shrew ABCA1 covered 7,762 bp, including a 6,786 bp coding region which encoded a 2,261 amino acids protein with the high identity to human ABCA1 (95%). Tree shrew ABCA1 was expressed in various tissues, the highest in lung, followed by liver, kidney, spleen and cardiac muscle in turn from high to medium expression levels. This pattern was partially different from that of human ABCA1 which was low in kidney and cardiac muscle. This work could shed new light on its role of ABCA1 in the distinctive HDL metabolism in tree shrew.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter 1
  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism*
  • Amino Acid Sequence
  • Animals
  • DNA, Complementary / genetics*
  • Gene Expression Regulation / genetics*
  • Humans
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Tupaiidae / genetics*
  • Tupaiidae / metabolism*

Substances

  • ABCA1 protein, human
  • ATP Binding Cassette Transporter 1
  • ATP-Binding Cassette Transporters
  • DNA, Complementary
  • RNA, Messenger