Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae

Nicoletta Guaragnella; Lucia Antonacci; Sergio Giannattasio; Ersilia Marra; Salvatore Passarella

doi:10.1016/j.febslet.2007.12.007

Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae

FEBS Lett. 2008 Jan 23;582(2):210-4. doi: 10.1016/j.febslet.2007.12.007. Epub 2007 Dec 17.

Authors

Nicoletta Guaragnella¹, Lucia Antonacci, Sergio Giannattasio, Ersilia Marra, Salvatore Passarella

Affiliation

¹ Dipartimento di Scienze per la Salute, Università del Molise, Via De Sanctis I, Campobasso, Italy.

PMID: 18082141
DOI: 10.1016/j.febslet.2007.12.007

Abstract

To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H2O2) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H2O2 levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H2O2 levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetic Acid / pharmacology*
Apoptosis / drug effects*
Catalase / metabolism*
Microscopy, Fluorescence
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / drug effects*
Saccharomyces cerevisiae / enzymology
Superoxide Dismutase / metabolism*

Substances

Catalase
Superoxide Dismutase
Acetic Acid