Abstract
The major histocompatibility complex (MHC) class II molecules HLA-DQ2 and HLA-DQ8 are key risk factors in coeliac disease, as they bind deamidated gluten peptides that are subsequently recognized by CD4+ T cells. Here, the production and crystallization of both HLA-DQ2 and HLA-DQ8 in complex with the deamidated gliadin peptides DQ2 alpha-I (PQPELPYPQ) and DQ8 alpha-I (EGSFQPSQE), respectively, are reported.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Celiac Disease / metabolism*
-
Cloning, Molecular
-
Crystallization
-
Crystallography, X-Ray
-
Deamination
-
Gliadin / chemistry
-
Gliadin / metabolism*
-
HLA-DQ Antigens / chemistry
-
HLA-DQ Antigens / genetics
-
HLA-DQ Antigens / isolation & purification
-
HLA-DQ Antigens / metabolism*
-
Humans
-
Leukocytes / chemistry
-
Leukocytes / metabolism*
-
Peptides / chemistry
-
Peptides / metabolism*
-
Protein Binding
Substances
-
HLA-DQ Antigens
-
HLA-DQ2 antigen
-
HLA-DQ8 antigen
-
Peptides
-
Gliadin