Tracking transmitter-gated P2X cation channel activation in vitro and in vivo

Nat Methods. 2008 Jan;5(1):87-93. doi: 10.1038/nmeth1144. Epub 2007 Dec 16.


We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The method has several advantages over previous attempts to image P2X channel activation by fluorescence resonance energy transfer (FRET): notably, it reports channel opening rather than a conformation change in the receptor protein. Our FRET-based imaging approach can be used as a general method to track, in real time, the location, regional expression variation, mobility and activation of transmitter-gated P2X channels in living neurons in vitro and in vivo. This approach should help to determine when, where and how different receptors are activated during physiological processes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Fluorescence Resonance Energy Transfer / methods*
  • Ion Channel Gating / physiology*
  • Neurons / physiology*
  • Receptors, Purinergic P2 / metabolism*
  • Receptors, Purinergic P2X


  • Receptors, Purinergic P2
  • Receptors, Purinergic P2X
  • Calcium