Identification of the Proteins, Including MAGEG1, That Make Up the Human SMC5-6 Protein Complex

Mol Cell Biol. 2008 Feb;28(4):1197-206. doi: 10.1128/MCB.00767-07. Epub 2007 Dec 17.

Abstract

The SMC protein complexes play important roles in chromosome dynamics. The function of the SMC5-6 complex remains unclear, though it is involved in resolution of different DNA structures by recombination. We have now identified and characterized the four non-SMC components of the human complex and in particular demonstrated that the MAGEG1 protein is part of this complex. MAGE proteins play important but as yet undefined roles in carcinogenesis, apoptosis, and brain development. We show that, with the exception of the SUMO ligase hMMS21/hNSE2, depletion of any of the components results in degradation of all the other components. Depletion also confers sensitivity to methyl methanesulfonate. Several of the components are modified by sumoylation and ubiquitination.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / metabolism*
  • Cell Survival / drug effects
  • Chromosomal Proteins, Non-Histone
  • DNA Damage
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Ligases / chemistry
  • Ligases / metabolism*
  • Mass Spectrometry
  • Methyl Methanesulfonate / pharmacology
  • Molecular Sequence Data
  • Protein Processing, Post-Translational / drug effects
  • Schizosaccharomyces pombe Proteins / chemistry
  • Schizosaccharomyces pombe Proteins / metabolism
  • Small Ubiquitin-Related Modifier Proteins / metabolism
  • Thermodynamics
  • Ubiquitination / drug effects

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Intracellular Signaling Peptides and Proteins
  • NSMCE3 protein, human
  • Nse4 protein, S pombe
  • SMC5 protein, human
  • SMC6 protein, human
  • Schizosaccharomyces pombe Proteins
  • Small Ubiquitin-Related Modifier Proteins
  • Methyl Methanesulfonate
  • Ligases
  • NSMCE2 protein, human