[Effect of Ganoderma lucidum polysaccharides peptide on invasion of human lung carcinoma cells in vitro]

Beijing Da Xue Xue Bao Yi Xue Ban. 2007 Dec 18;39(6):653-6.
[Article in Chinese]


Objective: To find out the effects of Ganoderma lucidum polysaccharides peptide (Gl-PP) on the invasion of the human lung carcinoma cell (PG cell).

Methods: PG cells were pretreated with different concentration Gl-PP in vitro, using cell proliferation assay, cell migration assay, adhesion assay, zymography and RT-PCR, then the effects of Gl-PP on proliferation, motility, adhesion and MMP-9 activity and mRNA expression of PG cells were investigated in vitro.

Results: Gl-PP did not directly inhibit PG cell proliferation in vitro. However, pretreated with Gl-PP, PG cells motility was inhibited significantly. PG cells adhesion was also inhibited. The activity of MMP-9 was inhibited in a dose-dependent manner, and the inhibited ratio was 41.53% at a dose of 100 mg/L Gl-PP. The mRNA expression of MMP-9 of pretreated PG cells was inhibited.

Conclusion: Gl-PP could suppress invasion of human lung carcinoma cells in vitro.

MeSH terms

  • Cell Adhesion / drug effects
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects
  • Humans
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology
  • Matrix Metalloproteinase 9 / metabolism
  • Neoplasm Invasiveness
  • Proteoglycans / pharmacology*
  • Reishi*


  • Proteoglycans
  • polysaccharide peptide
  • MMP9 protein, human
  • Matrix Metalloproteinase 9