Reactivity pattern of 92 monoclonal antibodies with 15 human papillomavirus types

J Gen Virol. 2008 Jan;89(Pt 1):117-129. doi: 10.1099/vir.0.83145-0.


Most anti-human papillomavirus (HPV) capsid antibody assays are based on virus-like particles (VLP). We evaluated glutathione S-transferase (GST)-L1 fusion proteins as ELISA antigens for determining type specificity and cross-reactivity of 92 VLP-specific monoclonal antibodies (mAb) generated against nine mucosal alpha papillomavirus types of species 7, 9 and 10. The antibody panel included 25 new mAb, and 24 previously published mAb are further characterized. We determined the cross-reactivity patterns with 15 different HPV types representing 6 species (alpha1, 2, 4, 7, 9 and 10) and neutralization and cross-neutralization properties with HPV types 6, 11, 16, 18 and 45. Eighty-nine (97 %) of the antibodies including 34, 71 and 14 recognizing neutralizing, conformational and linear epitopes, respectively, reacted with the GST-L1 protein of the HPV type used as immunogen, with log titres ranging from 2.0 to 7.3. Of these 89 antibodies, 52 % were monotypic, 20 % showed intra-species and 28 % inter-species cross-reactivity. Log neutralization titres to the immunogen HPV ranged from 1.7 to 5.6. A single cross-neutralizing mAb (H6.L12) was found. ELISA titres were always higher than neutralization titres. All neutralizing epitopes were conformational and mostly type-specific. Our data show that bacterially expressed, affinity-purified GST-L1 fusion proteins display a broad variety of epitopes and thus are well suited for detection of HPV antibodies. Cross-reactivity is associated with linear as well as conformational epitopes. Distantly related mucosal and skin alpha papillomaviruses share some conformational epitopes and the phylogenetic L1-based species definition may not define a serological unit since no species-specific epitope was found.

MeSH terms

  • Alphapapillomavirus / classification
  • Alphapapillomavirus / genetics*
  • Alphapapillomavirus / immunology*
  • Antibodies, Monoclonal*
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Capsid Proteins / immunology
  • Cross Reactions
  • Epitopes / analysis
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 16 / immunology
  • Humans
  • Neutralization Tests
  • Oncogene Proteins, Viral / immunology


  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Capsid Proteins
  • Epitopes
  • Oncogene Proteins, Viral
  • L1 protein, Human papillomavirus type 16