Evaluation of 5'-MGB hybridization probes for detection of Bordetella pertussis and Bordetella parapertussis using different real-time PCR instruments

Diagn Microbiol Infect Dis. 2008 Apr;60(4):429-32. doi: 10.1016/j.diagmicrobio.2007.10.019. Epub 2008 Feb 21.

Abstract

Rapid and accurate tests capable of distinguishing Bordetella pertussis from milder Bordetella parapertussis infection can aid in treating patients. We evaluated a novel real-time polymerase chain reaction (PCR) method that allows rapid and accurate diagnosis and distinction between B. pertussis and B. parapertussis infections. The method is based on a fluorescent 5'-minor groove-binding molecule hybridization probe that can clearly distinguish between B. pertussis and B. parapertussis by post-PCR melt curve analysis. The reagents worked equally well in several different real-time PCR instruments. The Bordetella detection reagents are combined with internal control components to account for PCR inhibition without compromising assay sensitivity.

Publication types

  • Evaluation Study

MeSH terms

  • Bordetella Infections / diagnosis
  • Bordetella parapertussis / genetics
  • Bordetella parapertussis / isolation & purification*
  • Bordetella pertussis / genetics
  • Bordetella pertussis / isolation & purification*
  • DNA, Bacterial / genetics
  • Diagnosis, Differential
  • Humans
  • Oligonucleotide Probes / genetics*
  • Polymerase Chain Reaction / methods*
  • Transition Temperature
  • Whooping Cough / diagnosis

Substances

  • DNA, Bacterial
  • Oligonucleotide Probes