Combining X-ray and electron-microscopy data to solve crystal structures

Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):70-5. doi: 10.1107/S0907444907053334. Epub 2007 Dec 5.

Abstract

Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement. The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / enzymology
  • Cryoelectron Microscopy / methods
  • Crystallography, X-Ray / methods*
  • Dimerization
  • Glycogen Synthase / chemistry
  • Glycogen Synthase / ultrastructure
  • Microscopy, Electron, Transmission / methods*
  • Models, Molecular
  • Software
  • Viral Structural Proteins / chemistry
  • Viral Structural Proteins / ultrastructure

Substances

  • VP2 protein, infectious bursal disease virus
  • Viral Structural Proteins
  • Glycogen Synthase