Direct Sequencing of Double-Stranded Polymerase Chain Reaction-Amplified 16S rDNA

Anal Biochem. 1991 Dec;199(2):216-8. doi: 10.1016/0003-2697(91)90092-8.

Abstract

A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Ribosomal / biosynthesis
  • DNA, Ribosomal / chemistry*
  • DNA, Ribosomal / isolation & purification
  • DNA, Single-Stranded / metabolism
  • DNA-Directed DNA Polymerase / metabolism
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Taq Polymerase

Substances

  • DNA, Ribosomal
  • DNA, Single-Stranded
  • Taq Polymerase
  • DNA-Directed DNA Polymerase